Fig. 7

APN conferred protective effects through SIRT1- PINK1-dependent mitophagy in PMVECs subjected to diabetic IR injury. A Mitochondrial membrane potential visualized by JC-1(scale bars, 20 μm). B The amount of ROS in PMVECs was determined by MitoSOX (scale bars, 20 μm). C Quantification of JC-1 fluorescence intensity (red/green florescent area) (mean ± SD). D Quantification of MitoSOX fluorescence intensity (red) (mean ± SD). E Mitochondrial ultrastructure. Mitochondria (red arrows) and mitophagosome (white arrows) in PMVECs were imaged with transmission electron microscopy (scale bars, 2 μm). F Flameng score (medians, interquartile range). G Cell apoptosis was determined by flow cytometry in rat PMVECs (medians, interquartile range). HF high fat, HG high glucose, SIR simulated ischemia–reperfusion. (^ccP < 0.05 versus the HG/HG + SIR group, ^ddP < 0.05 versus the HG/HG + SIR + A group)