Fig. 3

SIRT1 signaling pathway-mediated mitophagy playes an essential role in the antiapoptotic, anti-inflammatory and antioxidative effects of APN following diabetic lung IR injury. A Representative in situ detection of lung parenchymal cell apoptosis by TUNEL staining (scale bars, 200 μm). B Percentage of TUNEL-positive nuclei (medians, interquartile range). C Determination of mitochondrial membrane potential (medians, interquartile range). D Serum concentrations of interleukin-6 (IL-6) (mean ± SD). E Serum concentrations of TNF-α (mean ± SD). F lung concentrations of SOD (mean ± SD). G lung concentrations of MDA (mean ± SD). IL interleukin, MDA malondialdehyde, MMP mitochondrial membrane potential, SOD superoxide dismutase, TNF-α tumor necrosis factor-α, TUNEL terminal deoxynucleotidyl transferase dUTP nick end-labeling. (^aP < 0.05 versus the Con + Sham group, ^bP < 0.05 versus the Con + IR group, ^cP < 0.05 versus the DM + IR group, ^dP < 0.05 versus the DM + IR + A group; n = 8 in each group)