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Fig. 4 | Respiratory Research

Fig. 4

From: Mechanosensitive cation channel Piezo1 contributes to ventilator-induced lung injury by activating RhoA/ROCK1 in rats

Fig. 4

Blocking increased Piezo1 expression inhibited RhoA/ROCK1 pathway activation in CS-treated HPMECs. A Western blotting showed Piezo1, ROCK1, RhoA, and GAPDH levels in ad-scramble + sham, ad-scramble + CS, ad-siRNA + CS, and ad-siRNA + sham cells. Quantitative analysis of Piezo1 B and ROCK1 E mRNA expression using qPCR in the above cells (n = 3). Quantitative analysis of Piezo1 C and ROCK1 F protein levels in the above cells (n = 3). D Active GTP-RhoA was assessed using a pull-down assay in the above cells (n = 3). G Quantitative analysis of ROCK1 mRNA expression using qPCR in the sham, ad-Yoda1, ad-Yoda1 + siRNA, and ad-siRNA cells (n = 3). H Quantitative analysis of ROCK1 mRNA expression using qPCR in sham, CS, ad-GSMTx4 + CS, and ad-GSMTx4 cells (n = 3). Data are shown as the mean ± SEM. *P < 0.05 versus the sham group and #P < 0.05 versus the scramble + CS or Yoda1 group. CS cyclic stretch, HPMEC human pulmonary microvascular endothelial cell, siRNA small interfering RNA, qPCR quantitative real-time PCR

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