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Fig. 5 | Respiratory Research

Fig. 5

From: Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis

Fig. 5

Circ_PIP5K1A knockdown downregulated the ROCK1 expression by releasing miR-493-5p. A the online starbase indicate the binding sites between miR-493-5p and ROCK1 3′UTR. B, C the combination between miR-493-5p and ROCK1 3′UTR was analyzed through dual-luciferase reporter assay. D the transfection efficiencies of miR-493-5p and anti-miR-493-5p were assessed using RT-qPCR. E, F the effects of miR-493-5p overexpression or inhibition on ROCK1 mRNA (E) and protein (F) levels were detected via RT-qPCR and Western blot. GJ RT-qPCR and Western blot were performed for ROCK1 detection in DDP-resistant cells (G, H) and tumor-resistant tissues (I, J). K, L the correlations between ROCK1 and miR-493-5p (K) or circ_PIP5K1A (L) in tumor-resistant tissues were analyzed using Pearson’s correlation coefficient. M, N ROCK1 mRNA (M) and protein (N) levels were assayed by RT-qPCR and Western blot in A549/DDP and H460/DDP cells transfected with si-NC, si-circ_PIP5K1A, si-circ_PIP5K1A + anti-miR-NC or si-circ_PIP5K1A + anti-miR-493-5p. **P < 0.01, ***P < 0.001

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