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Fig. 2 | Respiratory Research

Fig. 2

From: Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis

Fig. 2

Circ_PIP5K1A knockdown inhibited DDP resistance and malignant behaviors in DDP-resistant NSCLC cells. Transfection of si-circ_PIP5K1A or si-NC was performed in A549/DDP and H460/DDP cells. A, B RT-qPCR was used to detect the levels of circ_PIP5K1A and PIP5K1A. C, D MTT assay was used to determine the IC50 value of DDP. E–G colony formation assay (E) and MTT (F, G) were used to assess cell proliferation capacity and cell viability. H–J flow cytometry was applied to examine cell cycle progression (H, I) and apoptosis (J). K, L transwell assay was applied to measure cell migration (K) and invasion (L). ***P < 0.001

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Respiratory Research

ISSN: 1465-993X

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