Fig. 2From: The Elk1/MMP-9 axis regulates E-cadherin and occludin in ventilator-induced lung injuryHigh tidal volume mechanical ventilation induced Elk1 and MMP-9 activation and occludin and E-cadherin degradation in C57BL/6 mice. C57BL/6 mice were treated with high tidal volume (20 mL/kg) for 0, 2, and 4 h. A Representative Western blotting of E-cadherin, occludin, Elk1, and MMP-9. B–E The density of the proteins at 0 h was used as a standard. *P < 0.05, vs. the MV(0 h) group. F Immunofluorescence was used to detected the distribution of occludin (green) and E-cadherin (red), and the nuclei were stained with DAPI (blue). All experiments were repeated at five timesBack to article page