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Fig. 9 | Respiratory Research

Fig. 9

From: TIPE2 inhibits PDGF-BB-induced phenotype switching in airway smooth muscle cells through the PI3K/Akt signaling pathway

Fig. 9

LY294002 reversed the effect of TIPE2 knockdown on ASMC phenotype switching. ASMCs were transfected with si-TIPE2 for 48 h in the presence of LY294002 (10 μM) and then treated with PDGF-BB. A The expression levels of PI3K and p-PI3K in ASMCs were determined by Western blotting. B Cell proliferation of ASMCs was measured by the MTT assay. C Cell migration of ASMCs was determined using the transwell assay. D The protein expression levels of calponin and SM22α in ASMCs were determined by Western blotting. E, F Quantification analysis of calponin and SM22α. Control group, *p < 0.05, vs. the si-NC + PDGF-BB group, #p < 0.05, vs. the si-TIPE2 + PDGF-BB group, &p < 0.05

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Respiratory Research

ISSN: 1465-993X

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