Fig. 6

Neutralization of systemic IL-33 significantly reduces airway mucus production, cytokine and chemokine release following OVA and RSV challenge. A Female BALB/c mice were treated as before with OVA to induce allergic inflammation. Following the last OVA challenge mice were given and i.p. injection of anti-IL-33 monoclonal antibody (300 ug/kg) followed by RSV infection on day 22. On day 25, anti-IL-33 treated mice were given a second injection of anti-IL-33. B Representative lung sections from PAS stained (40× magnification) airways of one mouse from each treatment group. Line scale is 50 μm. C depicts the mean with standard error bars of PAS + airway epithelial cell mucin staining. N = 15 airways/groups. D–I BAL supernates were tested for IL-33, TSLP, IL-23, IL-5, IL-13, CCL22, CCL17, IL-17 and IL-22 proteins by ELISA. Bars represent pg/mL detected per samples ± SEM