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Fig. 2. | Respiratory Research

Fig. 2.

From: Inhibition of EZH2 prevents acute respiratory distress syndrome (ARDS)-associated pulmonary fibrosis by regulating the macrophage polarization phenotype

Fig. 2.

3-DZNeP inhibits M1 macrophage polarization while induces M2 macrophage differentiation in the BALF of LPS-induced pulmonary fibrosis. Monocytic cells were isolated from BALF on Day 14 after intratracheal LPS in the control, control + 3-DZNeP, ALI, ALI + 3-DZNeP groups. A The mRNA expression of M1 marker (iNOS) and M2 markers (Arg-1 and FIZZ-1) was measured by quantitative PCR. B Cell lysates were subjected to immunoblot analysis with specific antibodies against iNOS, FIZZ-1, Arg-1, EZH2, p-STAT1, SOCS3, p-STAT6, SOCS1, PPAR-γ and GAPDH. C Expression levels of iNOS, Arg-1 and FIZZ-1 were quantified by densitometry and normalized using GAPDH. D Expression levels of EZH2, p-STAT and SOCS3 were quantified by densitometry and normalized using GAPDH. E Expression levels of p-STAT6, SOCS1, PPAR-γ were quantified by densitometry and normalized using GAPDH. (F) The levels of IL-1β, IL-6, TNF-α and IL-10 were measured by ELISA in the BALF. G The expression level of let-7C was qualified by Real time PCR. All data are expressed as mean ± SEM. (n = 9–15/group, *P < 0.05 vs. control and 3-DZNeP group, #P < 0.05 vs. ALI group, @P < 0.05 vs. control group determined by one-way ANOVA for multiple group comparisons)

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