Fig. 1.

3-DZNeP ameliorates the lung injury and represses subsequent fibrosis in ALI/ARDS animal model. A Quantification of protein levels in the bronchial alveolar lavage (BAL) on Day 1 after intratracheal LPS in the control, control + 3-DZNeP, ALI, ALI + 3-DZNeP groups. B Evaluation of wet/dry weight ratio is compared among control, control + 3-DZNeP, ALI, ALI + 3-DZNeP groups. C Quantification of IL-1β, IL-6, TNF-a and IL-10 levels in the lung homogenates in the control, control + 3-DZNeP, ALI, ALI + 3-DZNeP groups. D Photomicrographs illustrate Masson trichrome staining to highlight collagen deposition on Day 3, Day 7 and Day 14 after treatments as indicated (n ≥ 3 in each group; images are representative examples, ×400 magnification). E The Masson trichrome–positive interstitial area (blue) relative to the whole area from 10 random fields was analyzed. F Quantification of hydroxyproline on Day 3, Day 7 and Day 14 after intratracheal LPS in different groups. G Lung tissue lysates were subjected to immunoblot analysis with specific antibodies against α-SMA, Collagen I, E-cadherin, EZH2, H3K27me3 and GAPDH. F Expression levels of indicated proteins were quantified by densitometry and normalized with GAPDH. All data are expressed as mean ± SEM. (n = 9–15/group, *P < 0.05 vs. control and 3-DZNeP group, ^#P < 0.05 vs. ALI group, determined by one-way ANOVA for multiple group comparisons)