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Fig. 2 | Respiratory Research

Fig. 2

From: Family with sequence similarity 13 member A mediates TGF-β1-induced EMT in small airway epithelium of patients with chronic obstructive pulmonary disease

Fig. 2

FAM13A expression in BEAS-2B cells stimulated by TGF-β1 and transfection efficiency of lentiviral vectors overexpressing FAM13A or small hairpin RNA (shRNA) against FAM13A. A BEAS-2B cells were stimulated with different concentrations of TGF-β1 (0, 3, 5, and 10 ng/mL) for 48 h. Western blot analysis was performed to measure FAM13A protein expression. B and C BEAS-2B cells were treated with 0 or 10 ng/mL TGF-β1 for 48 h. Immunocytochemical and immunofluorescent staining was conducted to detect FAM13A protein expression. Representative images are shown. Magnification × 200. D and E BEAS-2B cells were infected with lentiviral vectors expressing FAM13A or shRNA against FAM13A. Western blot analysis and quantitative real-time PCR were performed to measure protein and mRNA levels of FAM13A at 48–72 h after transfection. shRNA-target 2 shows the highest knockdown efficiency. Data are expressed as mean ± SD. n = 3 (technical replicates). The experiment was repeated three times

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Respiratory Research

ISSN: 1465-993X

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