Fig. 3

Immunohistochemical expression of CTACK and CCR10 and HE staining. The immunohistochemical expression of CTACK (C, G, K) and CCR-10 (D, H, L); hematoxylin and eosin (HE) staining (A, B, E, F, I, J) in idiopathic pulmonary fibrosis (IPF) (A–D, E–H) and control lung tissues (I–L). A, E, I are low power view and the black bar indicates 200 μm, B–D, F–H, J–L are high power view and the black bar indicates 50 μm. A is magnified in (B–D, E), E is magnified in (F–H, and I), and I is magnified in (J–L). Alveolar macrophages observed in the alveolar spaces, showing the positivity of both antibodies (arrows in B, J). Compared to the macrophages staining, alveolar epithelium were negative for the antibodies in the normal lung. In IPF lungs, CTACK and CCR-10 are expressed predominantly in type II alveolar epithelial cells (arrows heads in B) and peripheral bronchiolar epithelial cells (arrow heads in F). The CTACK and CCR-10 staining intensity in IPF lung tissue is stronger than that in control lung tissue