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Fig. 3 | Respiratory Research

Fig. 3

From: Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis

Fig. 3

TNFα released from macrophages in response to bacterial LPS, plays a key role in driving mucus production in bronchial epithelial cells. Intracellular MUC5B staining in whole-mounts of human bronchial epithelial cells, grown as ALI cultures. Conditions include: Unstimulated (Vehicle), direct stimulation with LPS, stimulation with conditioned media from LPS activated macrophages w/wo addition of a TNFα-neutralizing antibody or direct stimulation with 50 ng recombinant TNFα w/wo addition of a TNFα-neutralizing antibody. Data are presented as mean ± standard deviation from three independent experiments using conditioned media from three different AM donors and triplicate technical replicates

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