Fig. 5

Knockdown of SERPINB10 expression affects survival of Th2 cells. a representative light micrograph of lentivirus (LV)-transduced CD4 T cells (top and bottom panels are pictures of the same cells in fluorescence mode and normal light mode respectively). b and c flow cytometric data for green fluorescent protein (GFP)⁺ percentage of lentivirus-transduced CD4 T cells. d SERPINB10 transcript level in lentivirus-transduced CD4 T cells (fold change compared with ACTB housekeeping gene). e percentage of IFN-γ⁺ or IL-4⁺ polarized CD4 cells upon completion of Th1 and Th2 polarization. f percentage of IFN-γ⁺ or IL-4⁺ polarized CD4 cells after completion of polarization and rested for another 8 days. g flow cytometric histogram for negative control (solid histogram) or SERPINB10-knockdown (line) Th1 and Th2 cells after staining with AnnexinV. Percentage of AnnexinV-positive cells were shown in histograms. Data were pooled from three independent experiment; n = 4 wells per group for each independent experiment. Data were mean ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant