Fig. 3
From: SERPINB10 contributes to asthma by inhibiting the apoptosis of allergenic Th2 cells
Serpinb10-knockdown mice had diminished numbers of Th2 cells after challenge. a Gating strategy to identify Th cells and their apoptosis in mouse lungs. Live cells can be separated in a CD45+ and a CD45− cell population. Within the CD45+ cell population, gate for CD4 T cells (CD3+ CD4+). Within the CD4 T cells, gate for Th1 cells (T-bet+) and Th2 cells (GATA-3+). Within the Th1 cells and Th2 cells, gate for apoptosis cells (Active Caspase-3+) respectively. b The numbers of Th1 and Th2 cells in mouse lungs. c percentage of caspase-3-positive Th1 and Th2 cells in mouse lungs. Data were combined from three experiments. n = 12–14. *, P < 0.05.