Fig. 1

Adeno-associated virus against Serpinb10 can inhibit the expression of baseline and HDM-induced Serpinb10 in mouse lung tissues. a Serpinb10 transcript levels in mouse lungs after 2 weeks of AAV treatment were determined by quantitative PCR. b and c Serpinb10 protein levels in mouse lungs after 2 weeks of AAV treatment were determined by western blotting. d C57BL/6 J mice were sensitized with 10 μg HDM on days 0,1,2 and challenged with 10 μg HDM daily from days 8–12. Mice were euthanized on day 14 for analysis. Control mice received PBS i.n. for sensitization and challenge. Serpinb10 AAV or negative control AAV was administered to the airway of mice 2 weeks before sensitization. e Serpinb10 transcript levels in mouse lungs on day 14 were determined by quantitative PCR. f and g Serpinb10 protein levels in mouse lungs on day 14 were determined by western blotting. Data were combined from three experiments (n = 12–14). One-way ANOVA was used. *, P < 0.05; **, P < 0.01; ***, P < 0.001