Fig. 7

Female Balb/c mice were senitised with house dust mite (HDM) in complete Freunds adjuvant intraperitoneally on days 0 and 1 and subsequently challenged intracheally with saline (N = 6) or HDM in saline (N = 8) on days 14 and 15. a Next generation sequencing was performed on lung tissue and cell deconvolution performed using a DCQ algorithm. Heatmap of z-score transformed scores calculated by the digital cell quantication algorithm for all three different groups. High values correspond to predicted enrichment of the cell type indicated in the rows. Only cells which have a significant change in quantity between conditions are shown. Highest up-regulation (light orange) to highest down-regulation (dark purple). b Lung digestion and flow cytometry was performed on the single cell suspension. c Effect of oral prednisolone 10 mg/kg b.i.d. on the presence of γδ-T-cells in the lungs of HDM treated mice. Data are expressed as mean ± SEM. Statistical analysis was performed using a non-parametric Kruskal–Wallis test with Dunn’s multiple comparison (***P < 0.005)