Skip to main content
Fig. 5 | Respiratory Research

Fig. 5

From: RORγt inhibitors block both IL-17 and IL-22 conferring a potential advantage over anti-IL-17 alone to treat severe asthma

Fig. 5

Female Balb/c mice were sensitized with house dust mite (HDM) in complete Freunds adjuvant intraperitoneally on days 0 and 1 and subsequently challenged intratracheally with saline (N = 6) or HDM in saline (N = 8) on days 14 and 15. BIX119 was dosed twice daily on days 14 and 15 by oral gavage. Mice were sacrificed on day 16 and airway hyperresponsiveness to acetylcholine (a), pressure–volume loops (b), and bronchioalveolar lavage fluid neutrophils (c), IL-17 (d), IL-22 (e) and KC (f) were measured. The relationship between bronchioalveolar lavage fluid neutrophil count (plotted as percentage inhibition from the HDM-alone treatment group) and lung tissue IL-17 (g) and lung tissue IL-17 / IL-22 (plotted as percentage inhibition from the HDM-alone treatment group) and trough plasma exposure (plotted as multiple of the mouse whole blood IL-17 IC50) (h) is shown. Data are expressed as mean ± SEM. Statistical analysis was performed using a non-parametric Kruskal–Wallis test with Dunn’s multiple comparison (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001)

Back to article page