Fig. 3

Female Balb/c mice were challenged intratracheally with saline (N = 4) IL-17A alone (N = 8), IL-22 (N = 8) alone or with the combination (N = 8). After 1 h, airway hyperreactivity (a) and lung tissue CXCL1 (b) were measured. After 24 h, bronchioalveolar lavage fluid neutrophil count (c) was measured. Data are expressed as mean ± SEM. Statistical analysis was performed using a non-parametric Kruskal–Wallis test with Dunn’s multiple comparison (*P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001)