Fig. 1
From: Mechanical ventilation enhances Acinetobacter baumannii-induced lung injury through JNK pathways
Mechanical ventilation enhanced Acinetobacter baumannii (A.b.)-induced MPO activity and IL-6 expression in lung tissues. a Mice receiving instillation of 108 CFU of A.b. after ventilation demonstrated a significant increase of the MPO activity in the lungs compared with instillation of 108 CFU of A.b. b Expression of TNF-α, IL-1β and IL-6 mRNA in the lungs of WT mice receiving P.a. or A.b. instillation and ventilation. Levels of mRNA expression were detected by real-time PCR. WT mice were intranasal instilled with 1 × 106 CFU of live A.b. and received mechanical ventilation for 3 h at 2 days after bacterial instillation. c Histological examination of the lungs indicated MV after instillations of 106 A.b. caused significant neutrophil infiltration (arrowhead) and alveolar swelling (arrow) in the lungs. MPO myeloperoxidase, MV mechanical ventilation, BALF bronchoalveolar lavage fluid, TNF tumor necrosis factor, IL interleukin, CFU colony-forming unit, PCR polymerase chain reaction; WT wild type, AL alveolar lumen, wild type, NS normal saline. *P < 0.05, **P < 0.01