Fig. 6
TGF-β1-induced upregulation of fibronectin, Tpm1.6/1.7 and Tpm2.1 protein: inter-donor variability. Human lung fibroblasts were left untreated (control) or stimulated with TGF-β1 (2 ng/mL) for 0, 24 and 48 h. Western blotting was utilized to quantitate protein levels of: a fibronectin (compared to HSP-70 as a loading control); b Tpm2.1 (compared to α-tubulin as a loading control); c Tpm1.6/1.7 (compared to α-tubulin as a loading control)). Results shown are densitometric analysis (normalised to loading controls and expressed as fold increase compared to control at 0 h). Data are protein levels detected in human lung fibroblasts from each of the nine donors with mean represented as black bars. Statistical analysis was performed using two-way ANOVA then Bonferroni's multiple comparisons test (where * denotes a significant effect of TGF-β1 (P < 0.05))