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Fig. 2 | Respiratory Research

Fig. 2

From: Tropomyosin 2.1 collaborates with fibronectin to promote TGF-β1-induced contraction of human lung fibroblasts

Fig. 2

TGF-β1 increases fibronectin protein upregulation. Human lung fibroblasts were left untreated (control) or stimulated with TGF-β1 (2 ng/mL) for 0, 24 and 48 h. Fibronectin protein upregulation was detected by Western blotting (compared to HSP-70 as a loading control). Results are shown as: a a representative Western blot; b densitometric analysis of fibronectin protein upregulation (normalised with HSP-70 and expressed as fold increase compared to control at 0 h). Statistical analysis was performed using two-way ANOVA then Bonferroni's multiple comparisons test (where * denotes a significant effect of TGF-β1 on fibronectin protein upregulation (P < 0.05)). Data are mean ± SEM values from n = 7 human lung fibroblast primary cell cultures

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