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Fig. 2 | Respiratory Research

Fig. 2

From: Long-term cigarette smoke exposure dysregulates pulmonary T cell response and IFN-γ protection to influenza virus in mouse

Fig. 2

CS altered total CD4 + and CD8 + T cells and IAV-specific CD4 + and CD8 + T cells in the lung of IAV-infected mice. C57BL/6 mice were exposed to CS (CS) or not (NS) for 6 weeks in a smoke exposure chamber (Teague Enterprises), then CS-exposed mice and NS mice were intranasally inoculated with a sublethal dose of IAV (300 pfu/mouse). At 7 and 10 days post IAV infection, lung cells were isolated and stained for flow cytometry to determine the total CD4 + and CD8 + cell numbers (a). b In vivo determination of the numbers of CD8 + T cells binding H-2Db/NP366-374 tetramers and numbers of CD4 + T cells binding I-Ab/NP311-325 tetramers in the lung on days 7 and 10. Cells from lung were stained for flow cytometry to determine the numbers of CD8 + T cells binding H-2Db/NP366-374 tetramers and numbers of CD4 + T cells binding I-Ab/NP311-325 tetramers to identify IAV antigen-specific CD4 + and CD8 + T cells. c, d Ex vivo determination of the numbers of CD4 + and CD8 + T cells producing IFN-γ after incubation of lung cells with or without NP peptide. c CD4 + T cells producing IFN-γ after incubation of lung cells with or without NP311-325 peptide. d Numbers of CD8 + T cells producing IFN-γ after incubation of lung cells with or without NP366-374 peptide. Lung cells were isolated and activated ex vivo with IAV-specific peptides: I-Ab-binding NP311-325 and H-2Db-binding NP366-374, respectively. Bar graph represents mean ± SEM (n = 5). # denotes a significant difference for NS groups between day 7 and 10 (P < 0.05, by one-way ANOVA with Student–Newman–Keuls test). * denotes a significant difference between CS and NS groups at the same time point (P < 0.05)

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