Fig. 8

Effects of exogenous H₂O₂ and NAC intervention on the proliferation of PASMCs and AASMCs. a The effects of exogenous H₂O₂ on the proliferation of PASMCs was biphasic, the proliferation of PASMCs increased in a dose-dependent manner by 0 ~ 50 μM H₂O₂ and was gradually inhibited by 100 ~ 1000 μM H₂O₂. b The effects of exogenous H₂O₂ on the proliferation of AASMCs was biphasic, the proliferation of AASMCs increased in a dose-dependent manner by 0 ~ 100 μM H₂O₂ and was gradually inhibited by 200 ~ 1000 μM H₂O₂. a and b The cell growth of 0 μM H₂O₂ group was set as 100% and the proliferation of treated cells was calculated as a percentage of them. n = 8, Data are means ± S.D. **P < 0.01 or *P < 0.05 vs. 0 μM H₂O₂. c 5 mM and 10 mM NAC effectively inhibited the proliferation of PASMCs induced by 50 μM H₂O₂. **P < 0.01 vs. without any treatment group, ^##P < 0.01 or ^#P < 0.05 vs. treatment with 50 μM H₂O₂ alone group. d 5 mM and 10 mM NAC effectively inhibited the proliferation of AASMCs induced by 100 μM H₂O₂. *P < 0.05 vs. without any treatment group, ^##P < 0.01 or ^#P < 0.05 vs. treatment with 100 μM H₂O₂ alone group. (c and d) The cell growth of the group without any treatment was set as 100% and the proliferation of treated cells was calculated as a percentage of them. n = 8, Data are means ± S