Fig. 4
From: Effects of elevated CO2 levels on lung immune response to organic dust and lipopolysaccharide
mRNA expression in mouse lung tissue. Values given are fold change in mRNA compared to a control value. mRNA harvested from lungs of mice given saline, organic dust (OD), or lipopolysaccharide (LPS) intranasally and then exposed to either room air (approximately 500 ppm), 1000 ppm (1 K), or 5000 ppm (5 K) CO2. Tissue was tested for the receptor TLR2 (a), TLR4 (b), cytoplasmic protein A20 (c) and Hsp1A (d). Results show reductions in TLR2, and Hsp1A with LPS treated animals plus CO2, and general decreases in A20 and Hsp1A for OD plus CO2. Hsp1A was elevated with CO2 delivery to control, to date the only protein mRNA found to do this. Bar graphs represent averages and error bars the SEM. N = 3–5 mice/group. by “a” (treatment vs respective saline control), “b” (1000 ppm vs room air treatment), “c” (5000 ppm vs room air treatment) and “d” (5000 ppm vs 1000 ppm treatment)