Fig. 3
From: Effects of elevated CO2 levels on lung immune response to organic dust and lipopolysaccharide
Protein expression in BAL fluid. Values given are in OD reading (a) MCP-1, b MIP-2, c KC, d IL-6, e IL-1B, f TNF-a, g IL-5, h IL-4, i IL-33, j IL-13, and k IL-10. Mice were treated intranasally with saline, organic dust (OD), or lipopolysaccharide (LPS) intranasally and then exposed to either room air (~ 500 ppm), 1000 ppm (1 K), or 5000 ppm (5 K) CO2. BAL fluid was centrifuged, cells removed and tested by ELISA. Results show reductions in KC and MIP-2 with LPS treated animals plus CO2, and an increase in all three for OD plus 5000 ppm CO2. Bar graphs represent averages and error bars the SEM. N = 3–5 mice/group. by “a” (treatment vs respective saline control), “b” (1000 ppm vs room air treatment), “c” (5000 ppm vs room air treatment) and “d” (5000 ppm vs 1000 ppm treatment)