Fig. 2

Microarray analysis identifies functional targets of TBX2 activity in E14.5 lungs. a Pie-chart summarizing the results of 4 individual transcriptional profiling experiments by microarrays of E14.5 control and Tbx2-deficient lungs. b Intersection of the list of genes upregulated in the microarrays of E14.5 Tbx2-deficient lungs and the list of genes associated with TBX2 ChIP peaks in the E14.5 lung. c List of genes upregulated in the microarrays of E14.5 Tbx2-deficient lungs and having a TBX2 ChIP-peak. Shown are the average fold changes (avgFC) of the 4 individual microarray data sets. d Functional annotation analysis by MouseMine websoftware identifies functional enrichment of terms related to stress response and growth control in the set of 39 genes upregulated in the microarrays of E14.5 Tbx2-deficient lungs and having a TBX2 ChIP-peak. e RNA in situ hybridization analysis of Ccn4 and Il33 expression on sections of E14.5 control and Tbx2-deficient lungs. f Scheme depicting the genomic loci of Ccn4 and Il33. Binding peaks identified by ChIP-Seq analysis are indicated above. Black boxes indicate peaks further validated by ChIP-PCR. g ChIP-PCR-validation of peaks in Il33 and Ccn4 as indicated in (f). Lanes were loaded as indicated