Fig. 6

Effect of CREB inhibitor (CREB inh) on fibroblast proliferative response to basal cell conditioned medium. Primary normal human lung fibroblast (NHLF) were plated in triplicate wells and treated with conditioned media from each of 3 non-smoking donors obtained in the presence or absence of 1 μg/ml LPA. For each condition, conditioned medium collected from cultures that were naive or treated with vehicle control (DMSO) or 200 nM CREB inhibitor (666-15) were collected and transferred to NHLF that had been serum starved for 48 h. a Fibroblast growth. NHLF were counted after 24 h. Shown is data from BC of a single individual. b Summary data showing mean fibroblast cell proliferation exposed to BC conditioned media from 3 donors. Data are expressed as the mean value of the 3 donors ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001