Fig. 4

Systemic blockade of interleukin-4 receptor alpha (IL-4Rα) reduces house dust mite (HDM)-induced eosinophil recruitment to the airways. Bronchoalveolar lavage fluid (BALF) was obtained on day 8 post-infection from pandemic H1N1 (pH1N1)-infected mice. a–c The percentage of eosinophils was determined in a total of 200 counted cells, and was compared using two-tailed Student’s t-test. d–e Eotaxin levels in BALF supernatant were measured by a multiplex assay, and compared using the Mann–Whitney U test. Data are expressed as mean ± SEM. Blue/pink: intranasal phosphate-buffered saline (PBS) (n = 10) / intranasal HDM (n = 9); green/orange: intranasal HDM followed by prophylactic strategy of IL-4Rα blockade (n = 12) / IgG (n = 12); black/red: intranasal HDM followed by therapeutic strategy of IL-4Rα blockade (n = 12) / IgG (n = 10). **P < 0.01; ***P < 0.001; ****P < 0.0001; ns: not significant