Fig. 3

a–e: a Immunofluorescence staining in mouse lung sections: Immunofluorescence staining on mouse lungs was performed for various time points: 0 (no LPS), 9 and 24 h post-LPS in WT (WT, N = 2) and CD34 KO (N = 2) mice. Red: Gr-1, marker for polymorphonuclear cells or neutrophil granulocytes; Green: vWF, marker for endothelium and platelets; Blue: DAPI, staining for cell nucleus. Representative images from three independent experiments. Platelet aggregates can be seen (white arrows) as vWF positive (green stained) clusters in alveolar septal post-LPS exposure. B: Bronchiole; B.V.: Blood vessel, A.S.: Alveolar septum. Scale: 50 μm, inset 25 μm; Results from lung image quantification are expressed as individual values and median to describe the central value. Fluorescence intensities, for Gr1/vWF and DAPI, were quantified in a minimum of 3–5 regions from each image (N = 1–2) for WT and CD34 KO mice at 0, 9 and 24 h post-LPS exposure. b DAPI normalized Lung Gr1 fluorescence intensity (Lung Gr1 FI: DAPI FI); c. DAPI normalized Lung vWF fluorescence intensity (Lung vWF FI: DAPI FI); d Lung perimeter normalized Lung Gr1 fluorescence intensity (Lung Gr1 FI (a.u.)/μm of lung parenchyma); e Lung perimeter normalized Lung vWF fluorescence intensity (Lung vWF FI (a.u.)/μm of lung parenchyma). ^#Shows significant interaction effect, *Shows main effect of time and o shows main mouse strain effect. The significant group differences are shown by bridged bars carrying *sign. P < 0.05