Fig. 2
From: PGC-1α regulates airway epithelial barrier dysfunction induced by house dust mite
Expression of mitochondrial biogenesis markers in the lungs of HDM-treated mice. a C57BL/6 mice were intranasally exposed to PBS or HDM extract every other day for 2 weeks and the mice were sacrificed. Lung tissues were removed after fixation and immune-fluorescence staining was performed to investigate the expressions of mitochondrial biogenesis markers (PGC-1α and TFAM) and E-Cadherin in the lungs of the mice. The negative control indicates secondary antibody alone. Representative photographs are shown. Bars: 100 μm. b–d The mean fluorescence intensity of PGC-1α, TFAM, and E-cadherin were quantified by using ImageJ. Data are expressed as means ± SD (n = 4). **p < 0.01