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Fig. 4 | Respiratory Research

Fig. 4

From: Asthma-related inflammation promotes lung metastasis of breast cancer cells through CCL11–CCR3 pathway

Fig. 4

Measurement of CCR3 antagonist (SB-297006) effects on 4T1 cells migration and proliferation. a Quantification of 4T1 tumor cells chemotaxis, using Boyden chamber assay, supplemented with recCCL11 and CCR3 antagonist (FBS1%: n = 8. FBS 1% + RecCCL11: n = 6 and FBS 1% + RecCCL11 + SB: n = 5), after 16 h. b After 72 h, proliferation of 4T1 cells treated with recCCL11 and CCR3 antagonist was assessed using BrdU staining (FBS 1%: n = 16, FBS 1% + RecCCL11: n = 14 and FBS 1% + RecCCL11 + SB: n = 14). Results are expressed as mean ± SEM. *p < 0.05, ***p < 0.001

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