Fig. 3

Measurement of CCR3 on 4T1 cells and neutrophils by flow cytometry. a The dot plot diagrams represent typical apoptotic and necrotic 4T1 cell populations detected by Annexin V-FITC and PI staining. The lower left quadrants (Q3; 49.2%) of the panels show viable intact cells, which were negative for Annexin V-FITC binding and excluded PI staining (FITC-/PI-); the upper right quadrants (Q1; 7.5%, Q2; 32.1%) show nonviable, necrotic cells, which were positive for Annexin V-FITC binding and PI uptake (FITC + /PI +). The lower right quadrants (Q4; 11.2%) represent apoptotic cells, positive for Annexin V-FITC and negative for PI (FITC + /PI-). b Representative histograms for flow cytometric analysis of surface expression of CCR3 receptors on 4T1 cells. IgG: Isotype control. c Lung neutrophils of mice treated with intratracheal instillation of recCCL11, sorted by FACS Aria