Fig. 1

Inflammation generated by OVA challenge induces tumor cell dissemination to the lung parenchyma (n = 8/group). a Timeline of mice challenged with OVA and s.c injected with 4T1 cells. b Volume of subcutaneous primary tumor. c, d Tumor size quantification assessed by measuring the ratio between the area of tumor foci in lungs and total lung tissue area on 8 sections per mouse in each group. Scale bar: 2 mm (hematoxylin–eosin). Results are expressed as mean tumor area/lung area ± SEM and are representative of 2 individual experiments. OVA indicate ovalbumine; PBS, phosphate-buffered saline (e) representative BALF total cell of mice exposed to OVA and subcutaneously injected with 4T1 cells. Scale bar: 50 µm (hematoxylin–eosin). f Quantification of isolated neutrophils migrating to a gradient of recCCL11 during 4 h using Boyden chamber assay. Neutrophils migration was estimated on 16 random fields (20 ×) of triplicate wells.*p < 0.05