Fig. 4
From: Role of CXCL16 in BLM-induced epithelial–mesenchymal transition in human A549 cells
CXCL16 regulated BLM-induced EMT and ECM excretion in A549 cells. CXCL16 siRNA was transfected into A549 cells for 12 h, after treated the cells with 200 μg/ml BLM for 24 h. The mRNA levels of E-cad (a), α-SMA (b) and COLI (c) was determined by RT-PCR and the protein levels of E-cad (d), α-SMA (e) and COLI (f) was determined by western blot (g). BLM Bleomycin, E-cad E-cadherin, α-SMA α-smooth muscle actin, COLI collagen I; GAPDH served as a loading control. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant. Data represented three independent experiments