Fig. 1
From: Role of CXCL16 in BLM-induced epithelial–mesenchymal transition in human A549 cells
BLM-treated A549 cells have undergone EMT. A549 cells were incubated with different concentrations of BLM for 24 h and the MTS assay was used to detect cell viability (a). Levels of EMT-related markers were detected, the mRNA levels of E-cad (b) and α-SMA (c) were detected by real-time PCR, the protein levels of E-cad (e) and α-SMA (f) were detected by western blot (d). EMT epithelial–mesenchymal transition, BLM Bleomycin, E-cad E-cadherin, α-SMA α-smooth muscle actin; GAPDH served as a loading control; *P < 0.05, **P < 0.01, ***P < 0.001. Significantly different versus 0 μg/ml. Data represented three independent experiments