Fig. 2

S100A9 blockade ameliorates lung injury in LPS-treated mice. a Representative images of hematoxylin and eosin staining of the left lung (scale bar, 100 μm). b The lung injury score was determined based on histological analysis. c The lung wet-to-dry weight ratio is presented. d The total protein levels in BALF were measured using a BCA assay. e–f The MPO activity and LDH content in lungs were measured to evaluate oxidative injury. Data are expressed as the mean ± SEM (n = 6 for each group). Statistical analysis was performed using one-way ANOVA followed by Tukey's multiple comparison test. ^*P < 0.05, and ^**P < 0.01 vs. the control group; ^#P < 0.05, ^##P < 0.01 vs. the LPS group. S100A9 S100 calcium binding protein A9, LPS lipopolysaccharide, BALF bronchoalveolar lavage fluids, MPO myeloperoxidase, LDH lactate dehydrogenase