Fig. 3
From: Hermansky-Pudlak syndrome-2 alters mitochondrial homeostasis in the alveolar epithelium of the lung
Mitochondrial function is impaired in the HPS-2 alveolar epithelium. a Mitochondrial oxygen consumption in control and HPS-2 mouse lung epithelial (MLE) cells (n = 3, each group). b Total ATP levels in control and HPS-2-MLE15 cells (n = 4, each group). c Mitochondrial ROS levels in control and HPS-2-MLE15 cells (n = 6, each group). d Aconitase activity in control and HPS-2-MLE15 cells (n = 6, each group). e–g Glycolytic activity in control and HPS-2-MLE15 cells as measured by transcript levels for hexokinase and lactate dehydrogenase, lactate levels and extracellular acidification rate (ECAR) (n = 3–4, each group). h Immunoblot for total and phosphorylated AMPK levels in control and HPS-2- MLE15 cells (n = 4, each group). Densitometry measurements shown on the right. Student’s t-test. *P < 0.05, **P < 0.01 vs. control group