Table 1 List of antibodies used for western blot analysis
Primary and Secondary antibodies | Â | Size (kDa) | Code |
|---|---|---|---|
α-SMA | Monoclonal mouse anti-human smooth muscle actin, clone 1A4 | 43 | M0851, DAKP/Agilent |
GAPDH | D16H11 XP Rabbit mAb | 37 | 5174, Cell Signaling Technology, MA, USA |
Smad2/3 | D7G7 XP Rabbit mAb | 52, 60 | 8685, Cell Signaling Technology, MA, USA |
Smad 2 | D43B4 XP Rabbit mAb | 60 | 5339, Cell Signaling Technology, MA, USA |
Smad 3 | C67H9 Rabbit mAb | 52 | 9523, Cell Signaling Technology, MA, USA |
Phospho-Smad 2 (Ser465/467) | 138D4 Rabbit mAb | 60 | 3108, Cell Signaling Technology, MA, USA |
Phospho-Smad 3 (Ser423/425) | C25A9 Rabbit mAb | 52 | 9520, Cell Signaling Technology, MA, USA |
ERK1/2 (p44/42) MAPK | 137F5 Rabbit mAb | 46,54 | 4695, Cell Signaling Technology, MA, USA |
p38 MAPK | D13E1 XP Rabbit mAb | 40 | 8690, Cell Signaling Technology, MA, USA |
SAPK/JNK | Rabbit Ab | 46,54 | 9252, Cell Signaling Technology, MA, USA |
Phospho-ERK1/2 (p44/42) MAPK (Thr202/Tyr204) | D13.14.4E XP Rabbit mAb | 42, 44 | 4370, Cell Signaling Technology, MA, USA |
Phospho-p38 MAPK (Thr180/Tyr182) | D3F9 Xp Rabbit mAb | 40 | 4511, Cell Signaling Technology, MA, USA |
Phospho-SAPK/JNK (Thr 183/Tyr 185) | 81E11 Rabbit mAb | 46,54 | 4668, Cell Signaling Technology, MA, USA |
HO-1 | E3F4S Rabbit mAb | 28 | 43,966, Cell Signaling Technology, MA, USA |