Fig. 4

Effect of AITC and selective TRPA1 antagonist HC-030031 on cell migration after TGF-β1 stimulation. a–g Representative images under light microscope and h quantification of cell number in the gap area. Cells were manually scratched with a pipette tip, incubated with b AITC alone or c HC-030031 alone, and pretreated with d TGF-β1 (5 ng/ml) and incubated with e AITC (10 μM) or f HC-030031 (10 μM) or g AITC (10 μM) + HC-030031 (10 μM) for 24 h. TGF-β1 promoted migration of MRC-5 cells and AITC significantly inhibited the migration of TGF-β1-treated MRC-5 cells. HC-030031 did not hinder the migration of cells and was not able to reverse the suppressive effect of AITC (*P < 0.05). N = 7