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Fig. 3 | Respiratory Research

Fig. 3

From: AITC inhibits fibroblast-myofibroblast transition via TRPA1-independent MAPK and NRF2/HO-1 pathways and reverses corticosteroids insensitivity in human lung fibroblasts

Fig. 3

Effect of TRPA1 modulators on protein expression and immunoreactivity of α-SMA after TGF-β1 stimulation. a Western blot analysis of α-SMA protein expression after treatment with AITC (10 μM), HC-030031 (10 μM) or AITC (10 μM) + HC-030031 (10 μM) for 24 h in the latter with TGF-β1 incubation for 48 h where TGF-β1-induced α-SMA protein expression was decreased by AITC (*P < 0.05 at TGF vs TGF + AITC or TGF + AITC + HC). N = 5. b Immunohistochemical results showing α-SMA protein expression with stress fibers in cells after similar treatment to (a). (e) Summary of quantification of α-SMA positive cells. TGF-β1 increased α-SMA-immunoreactive cells and AITC significantly reduced number of the positive cells (*P < 0.05, * without bar; compared with control). N = 6

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