Fig. 2
From: Bitter taste receptor agonists regulate epithelial two-pore potassium channels via cAMP signaling
Denatonium benzoate-induced current changes are reversible by cAMP treatment. Representative Ussing chamber tracings of human sinonasal ALI cultures treated with 10 µM amiloride a followed by vehicle (dashed line) or 2 mM denatonium benzoate (solid line) and then a 100 µM IBMX/10 µM Forskolin (I/F) followed by 10 µM CFTRinh-172 (inh172) or b inh172 followed by I/F. These data demonstrate denatonium-induced current changes are reversible with an increase in cAMP (I/F treatment) even in the presence of inh172 and are therefore independent of CFTR function. c Representative tracing demonstrating that pretreatment with both inh172 and I/F to blocks denatonium-mediated current changes. d 100 ng/ml pertussis toxin pretreatment decreases denatonium (2 mM)-mediated change in Isc. Shown in d are the individual data points, mean and standard error of the mean