Fig. 7

Pretreatment with N-acetylcysteine (NAC) protects against CFTR dysfunction by secondhand smoke (SHS) in murine airways. a. Representative Ussing chamber electrophysiologic tracing of excised trachea from mice exposed to either control room air or SHS for 6 weeks in nose-only inhalation system with or without NAC added to drinking water (40 mM). b. Summary graph illustrating CFTR activity following addition of forskolin (10 μM) + IBMX (100 μM) under an apical chloride ion gradient generation and presence of ENaC inhibitor amiloride. Subsequent bumetanide (10uM) inhibition of basolateral NKCC1, that generates chloride ions for CFTR channels, was used as a secondary confirmation of CFTR activity (c). n = 8–10, *P < 0.05