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Fig. 4 | Respiratory Research

Fig. 4

From: Evaluation of secondhand smoke effects on CFTR function in vivo

Fig. 4

Acrolein, a highly reactive aldehyde known to inhibit CFTR function, is present in secondhand smoke (SHS) and is neutralized by N-acetylcysteine (NAC). Acrolein content was estimated by exposing cell-free PBS to SHS for 10 min under conditions to which bronchial epithelial cells and mice were exposed. Exposure samples were immediately derivatized with dinitrophenylhydrazine to generate stable acrolein intermediates for mass spectrometry chemical analysis. Summary of acrolein measured in presence and absence of NAC (300 μM) added to the sample collection buffer is shown. n = 4/condition, *P < 0.05

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