Fig. 4

Antioxidant genes are blunted in parenchymal tissues of female mice and, Mmp12 and Cxcl2 genes are positively correlated with 3NTyr. a) Cyp1a1 and b) Nqo-1, c) Mmp12 and d) Cxcl2 gene expression was normalized to β2m in parenchymal tissues of male, female and ovariectomized mice exposed to air (control, C) or smoke (S) for 6 months. Values were expressed as mean ± SEM (n = 4–5). E) Mmp12 and F) Cxcl2 gene expression was correlated with 3-nitrotyrosine (3NTyr) per mg of total protein. One-way analysis of variance with Bonferroni’s multiple comparisons test was used in panels a-d. Linear regression analyses were used panels E-F. Cyp1a1 = cytochrome p450; Nqo1 = NAD(P)H:quinone oxidoreductase 1, Mmp12 = matrix metalloproteinase, Cxcl2 = chemokine ligand 2 (macrophage inflammatory protein 2-alpha); β2m = β2-macroglobulin.