Fig. 1From: Sex differences in lymphoid follicles in COPD airwaysFemale mice have more lymphoid follicles than male mice after chronic smoke exposure, and this effect was attenuated by ovariectomy. Representative images of a) vessel-associated, b) bronchial-associated and c) parenchymal-associated lymphoid aggregates in lung cross sections from smoke-exposed female mice were stained with H&E. Scale bar = 100 μm. The number of lymphoid aggregates per area (cm2) was quantified and compartmentalized (vessel-, bronchial- and parenchymal-associated) in lung sections of air-exposed (control) and smoke-exposed d) male, e) female and f) ovariectomized mice. g) Fractional area of lymphoid aggregates was quantified and normalized to the total lung cross-sectional area using the Aperio Imaging system. The number of lymphoid aggregates per area (cm2) was quantified and compartmentalized (vessel-, bronchial- and parenchymal-associated) in lung sections of H) air-exposed (control) male vs. female mice, and I) smoke-exposed male vs female mice. Values were expressed as mean ± SEM with N = 5–7 per group. Parametric t-test was performed in panels D-F and H-I. One-way ANOVA with Bonferroni’s multiple comparisons tests were performed in panel G.Back to article page