Fig. 1
Expression levels of phagocytic receptors on human alveolar macrophages. a FACS gating strategy to delineate alveolar macrophages. Cell suspension was collected by perfusing and lavaging human lung tissues with saline. Alveolar macrophages were detected as live, FSChi/SSChi, single cell-gated CD45+/CD206+ cells in the cell suspension. b Representative histograms for the expression levels of FcγRI, CD11b, macrophage scavenger receptor-1 (MSR-1), CD36 and Siglec-1, on FSChi/SSChi/CD45+/CD206+ alveolar macrophages of control never-smokers (CNS, n = 11), control ex-smokers (CES, n = 9) and COPD ex-smokers (COPD, n = 11). Specific staining, open; isotype control, shaded