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Fig. 7 | Respiratory Research

Fig. 7

From: Lung-protective ventilation worsens ventilator-induced diaphragm atrophy and weakness

Fig. 7

LPV downregulated PGC–1α expressions in the diaphragm. (a), the heatmap is generated by selecting differentially expressed genes. These genes could be separated into three major cluster (C1, C2, and C3). The C1 cluster contains up–regulated genes in LPV that are reversed in CV; the C2 cluster contains down–regulated genes in LPV that are mostly reversed in CV except C2.1 which contains down–regulated genes in both LPV and CV; the C3 cluster contains down–regulated genes in LPV that are overly reversed (C3.1, higher than CON) and up–regulated genes in both LPV and CV; (b), fold changes in Ppargc1a (PGC–1α) gene expression between the LPV and CV groups; (c), western blots for PGC–1α protein expressions in the diaphragm. RNA–seq and western blots suggested that PGC–1α protein expressions were significantly decreased in the LPV group as compared with the CV group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. CON group; ##p < 0.01, ###p < 0.001 vs. CV group

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