Downregulation of hsa-microRNA-204-5p and identification of its potential regulatory network in non-small cell lung cancer: RT-qPCR, bioinformatic- and meta-analyses

Table 2 Clinicopathological parameters and the expression of miR-204-5p in LUAD. Annotation: LUAD, lung adenocarcinoma. a, paired sample’s t test performed to compare miR-204-5p expression between NSCLC and the controls; Independent sample’s t test processed to assess relationships between miR-30d-5p expression and the clinicopathological parameters of NSCLC. TNM, tumor, node, metastasis; b, One-way ANOVA preformed to evaluate distributive feature of miR-204-5p in three or more groups of clinicopathological parameters

Clinicopathological parameters		n	Relevant expression of miR-204-5p (2^−ΔCq)
Clinicopathological parameters		n	Mean ± SD	t/F-value	p-value
Tissue	LUAD	101	3.4663 ± 1.82397	-2.731^a	0.007
Tissue	Non-cancer	101	4.2786 ± 2.36824	-2.731^a	0.007
Gender	Male	56	3.7768 ± 1.91937	1.934	0.056
Gender	Female	45	3.0800 ± 1.63729	1.934	0.056
Age (years)	< 60	41	3.7390 ± 1.85039	1.245	0.216
Age (years)	> = 60	60	3.2800 ± 1.79734	1.245	0.216
Smoke	No	26	4.1000 ± 1.67141	−0.695	0.491
Smoke	Yes	18	4.4611 ± 1.72768	−0.695	0.491
Tumor size	<=3 cm	53	3.0906 ± 1.72362	−2.218	0.029
Tumor size	> 3 cm	48	3.8813 ± 1.85915	−2.218	0.029
Vascular invasion	No	70	4.1114 ± 1.63215	6.286	< 0.001
Vascular invasion	Yes	31	2.0097 ± 1.34123	6.286	< 0.001
TNM	I-II	44	3.8864 ± 1.87190	2.066	0.041
TNM	III-IV	57	3.1421 ± 1.73339	2.066	0.041
Lymph node metastasis	No	45	4.0556 ± 1.86822	3.027	0.003
Lymph node metastasis	Yes	56	2.9929 ± 1.65660	3.027	0.003
Pathological grading	I	17	4.2176 ± 1.94140	5.477^b	0.006
	II	61	3.6279 ± 1.81752
	III	23	2.4826 ± 1.36070

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