Fig. 4

The VGVAPG elastin peptide affect IL-4 expression in Th2 cells from COPD patients. CD4⁺ T-cells isolated from COPD patients (n = 29) and controls (13) were stimulated with anti-CD3/CD28 beads in the presence or not of the VGVAPG peptide (10 µg/mL). After 48 h incubation, stimulated cells were collected, stained and analyzed by flow cytometry to evaluate the levels of a IL-4, b IFN-γ and c IL-17A intracellular cytokine expression. *p < 0.05. d Quantification of IL-4 production in culture supernatants of anti-CD3/CD28-stimulated CD4⁺ T-cells in the presence or not of the VGVAPG peptide (10 µg/mL). Quantification was performed by Cytometric Bead Array Analysis. Data are presented as mean ± SD. *p < 0.05